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不同灵芝菌株菌丝体乙醇提取物体外抑瘤活性的比较和机理

来源:食用菌网 作者:易菇网 人气: 发布时间:2018-08-20
摘要:不同灵芝菌株菌丝体乙醇提取物体外抑瘤活性的比较和机理ACOMPARISONSTUDYOFTHEINVITROANTICANCERACTIVITYANDMECHAN
不同灵芝菌株菌丝体乙醇提取物体外抑瘤活性的比较和机理 A COMPARISON STUDY OF THE IN VITROANTICANCER ACTIVITY AND MECHANISM OF ETHANOL EXTRACTS FROM MYCELIA OF DIFFERENT GANODERMA LUCIDUM STRAINS 杨晓彤  周月琴  李绪全  冯慧琴  糜可  杨庆尧  摘 要:以人急性早幼粒白血病细胞HL-60细胞株作为模型,用MTT法测定生长抑制率,流式细胞Annexin V实验和DNA含量法研究抑瘤机制,比较了8个灵芝Ganodermalucidum菌株发酵菌丝体乙醇提取物的体外抗肿瘤活性和抑瘤机制.筛选结果得到了能产生高抑瘤活性乙醇提取物的灵芝菌株L5.其对HL-60细胞的抑制率为91.4±0.9%(72小时,125μg/mL);作用48小时后,13.3%的细胞发生早期凋亡,G0/G1期细胞比例比对照组增加15.9%,而S期细胞比例则下降了8.4%,G2/M期细胞数减少7.6%.本研究证明了灵芝菌丝体乙醇提取物能有效抑制HL-60肿瘤细胞的体外生长,抑制率与菌株相关,其抑瘤机制与细胞Go/G1期阻滞和诱导凋亡有关. 关键词:细胞周期,凋亡,HL-60细胞 分类号:Q936 文献标识码:A 文章编号:1672-6472(2005)02-0251-0258 基金项目:上海市教育委员会科学项目资助(项目号04DB20) 作者简介:杨晓彤,通讯作者:email:xtyang@shnu.edu.cn 作者单位:杨晓彤(上海师范大学微生物与免疫学研究所,上海,200234)       周月琴(上海师范大学微生物与免疫学研究所,上海,200234)       李绪全(上海师范大学微生物与免疫学研究所,上海,200234)       冯慧琴(上海师范大学微生物与免疫学研究所,上海,200234)       糜可(上海师范大学微生物与免疫学研究所,上海,200234)       杨庆尧(上海师范大学微生物与免疫学研究所,上海,200234)  参考文献: [1]Cai H, Wang FS, Wang LF, Bai Y, Xu L, Yang JS, Zhang YM, 2001. 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